Yingqiang Wen

Ph.D. & Professor

Chair of Department of Pomology

College of Horticulture, Northwest A&F University

No.3 Taicheng Road

Yangling, Shaanxi Province, 712100, P. R. China

Tel: 86-29-87082613(O)

Email: Wenyq@nwsuaf.edu.cn

Education

2011, Ph.D., Biochemistry and Molecular Biology, Northwest A&F University, China

11/2007-09/2010, Visiting PhD Student, Center for Biosystems Research (Now name is The Institute for Bioscience and Biotechnology Research), University of Maryland, USA

2002, M.S., Pomology, Northwest A&F University, China

1997, B.S., Pomology, Northwest Agricultural University, China

Professional Experience

07/2019~07/2020: Visiting Professor, Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, USA

08/2016~12/2016: Visiting Professor, Division of Viticulture and Pomology, University of Natural Resources and Life Sciences, Vienna, Austria

01/2016~present, Professor, College of Horticulture, Northwest A&F University, China

01/2015~02/2015: Visiting Scholar, School of Agriculture, Food and Wine, The University of Adelaide, Australia

01/2011~12/2015, Associate Professor, College of Horticulture, Northwest A&F University, China

01/2003~12/2010, Instructor, College of Horticulture, Northwest A&F University, China

06/1997~12/2002, Teaching Assistant, Department of Horticulture, Northwest Agricultural University, China

Professional Memberships

the International Society for Horticultural Science

the Chinese Society for Horticultural Science

Shaanxi Society for Horticultural Science

Honors and Awards

2020, One article (As correspondence author) was selected as Top Articles in outstanding S&T journal of China, Frontrummer 5000

2015, Excellent supervisor for master student, College of Horticulture, Northwest A&F University, China

2011, Third Grade Award of Young teacher lecture competition, Northwest A&F University, China

2011, First Grade Award of Young teacher lecture competition, College of Horticulture, Northwest A&F University, China

2007, First Grade of Shaanxi province Science and Technology Award, Shaanxi province, China, Rank eighth

2005, First Grade of Teaching achievement Award, Northwest A&F University, China, Rank second

Research Interests

1) Molecular breeding for grapevine while integrating novel techniques.

Novel techniques in plant breeding are being developed for potential contributions to grapevine improvement. We have developed an effective Agrobacterium-mediated transformation using embryogenic  Vitis  cultures. Genes encoding antifungal compounds are being transferred to important commercial cultivars for the improvement of disease resistance. We also targeted some susceptible genes using CRISPR/Cas9 technology in order to increase resistance to powdery mildew in grape cultivar.

2) The genetic improvement of grapevine while integrating traditional techniques.

The interspecific hybridization is used to develop table grapes with early ripening, high yield, disease resistance and drought tolerance quality. Disease resistance is a high priority objective. Our best accessions, usually derived from native Chinese species, are used as parents to impart high levels of disease resistance to table grape progenies.

Teaching Responsibilities

• Taught 8 weeks course every autumn on General Cultivation Science of Fruit Tree for Horticulture 3034114. 2003-2007, 2011-2017.

• Taught 6 weeks course every spring on Cultivation Science of Deciduous Fruit Tree for Horticulture 3034115. 2003-2007, 2011-2017.

• Taught 8 weeks course every autumn on Fruit Tree Breeding for Horticulture 4034116. Begin in 2018.

• Guide graduate students in the fields of Horticulture.

Graduate Field Memberships

Fruit Science

Service

College Service

• 2017-present   Chair of Department of Pomology

• 2019-present   Member, Professor Committee

• 2019          Member, Tenure Review Committee

Editorial boards

Editorial Board as a Topic Editor, Horticulturae, 2021-

Ad hoc Reviewing for Journals

Tree Genetics & Genomes, Plant Cell, Tissue and Organ Culture, Scientia Horticulturae, HortScience, Journal of Integrative Agriculture, PLOS ONE, Journal of Plant Physiology & Pathology, Horticultural Plant Journal, Plants, Biologia Plantarum, Horticulture, Environment, and Biotechnology, Plant Systematics and Evolution, Bioscience, Biotechnology, and Biochemistry, British Biotechnology Journal, Peer Journal, Journal of plant genetics resources (in Chinese), Journal of Northwest forestry university (in Chinese)

Reviewing for grant agencies

NSFC (2011-2019)

Invited Presentations

NATIONAL AND INTERNATIONAL MEETING TALKS

04/14/2019, Nanjing Agricultural University, China,  CRISPR/Cas9-mediated targeted mutagenesis in grapevine .

07/25/2018, Karlsruhe Institute of Technology, Germany,  Interaction of Powdery Mildew and Wild Chinese Grapevin  e .

04/14/2018, Annual Meeting of 4^th berry in China, Shanghai Jiaotong University, China,  Gene editing technology exploring in grapevine .

12/15/2016, Meeting of Eurasia-Pacific Uninet Scholars, University of Natural Resources and Life Sciences, Vienna, Austria,  Interaction between Grapevine and Pathogen/Pest .

10/21/2012, Annual Meeting of Chinese Society for Horticultural Science, Yangling, Shaanxi Province, China.  Functional analysis of a novel Aldehyde Dehydrogenase gene   VpALDH2B4   from Chinese Wild Vitis pseudoreticulata  .

08/19/2011, Annual Meeting of Chinese Society for Plant Pathology, Yichang, Hubei province, China.  Utilization of a Sow thistle powdery mildew as a poorly   adapted pathogen to dissect post-invasion nonhost resistance mechanisms in Arabidopsis  .

INVITED SEMINARS/TALKS

08/23/2019, Institute for bioscience and biotechnology research, University of Maryland, USA,  Grape breeding in North America .

07/27/2018, University of Verona, Italy,  Interaction of Powdery Mildew and Wild Chinese Grapevin  e .

03/05/2018, Institute of Fruit and Floriculture Research, Gansu Academy of Agricultural Sciences, China,  Functional analysis of resistance gene in  g rapevin  e .

12/11/2017, Forest and fruit tree Institute, Shanghai Academy of Agricultural Sciences, China,  Molecular and Cellular basis of the resistance to powdery mildew in   Wild Chinese Grapevin  e .

12/10/2017, School of Agriculture and Biology, Shanghai Jiaotong University, China,  Molecular and Cellular basis of the resistance to powdery mildew in   Wild Chinese Grapevin  e .

11/21/2017, Zhengzhou Fruit Research Institute, Chinese Academy of Agriculture Sciences, China,  Cellular mechanism between   Wild Chinese Grapevin  e and powdery mildew .

02/10/2017, Institute for bioscience and biotechnology research, University of Maryland, USA,  Cellular basis of the resistance to powdery mildew in   Wild Chinese Grapevin  e .

08/18/2016, Division of Viticulture and Pomology, University of Natural Resources and Life Sciences, Vienna, Austria,  Interaction of Powdery Mildew and Wild Chinese Grapevin  e .

03/18/2015, Institute of Fruit and Floriculture Research, Gansu Academy of Agricultural Sciences, China , The progress of   Wild Chinese Grapevin  e resistance to powdery mildew.

04/14/2012, Hybrid rape research center of Shaanxi province, Dali, Shaanxi,  Molecular mechanism of  Arabidopsis broad-spectrum resistance protein RPW8.2.

National & International Meeting (Posters & Abstracts)

07/27/2018, University of Verona, Italy.  Ectopic expression of Arabidopsis broad-spectrum resistance gene RPW8.2 improves the resistance to powdery mildew in grapevine (Vitis vinifera)  .  Poster

07/25/2018, Karlsruhe Institute of Technology, Germany.  Ectopic expression of Arabidopsis broad-spectrum resistance gene RPW8.2 improves the resistance to powdery mildew in grapevine (Vitis vinifera)  .  Poster

07/15-20/2018, XII International Conference on Grape Breeding and Genetics, Bordeaux, France.  Molecular improvement of powdery mildew resistance in grapevine , Abstract

10/27/2017, Huazhong Agricultural University, China.  Two grapevine CDPKs improve powdery mildew resistance via tuning MAPK-ACS-Ethylene signaling module in a dose-dependent manner.  Poster

10/27/2017, Huazhong Agricultural University, China.  Targeted editing   of   MLO   genes using   the   CRISPR/Cas9 system   trigger  s   cell death   and restricts   hyphal length   infection of powdery mildew in grapevine (Vitis vinifera)  . Poster

03/25/2010, Mid-Atlantic Section American Society of the Plant Biologists Annual Spring Meeting, College Park, USA.  Basal Resistance Mechanisms Revealed by  Golovinomyces cichoracearum UMSG1, A Poorly Adapted Powdery Mildew Pathogen in Arabidopsis . Poster

03/25/2009, Mid-Atlantic Section American Society of the Plant Biologists Annual Spring Meeting, College Park, USA.  Functional Diversification between RPW8.1 and RPW8.2, Two Homologous Disease Resistance Proteins in Arabidopsis thaliana . Poster

Patents

1. Wen Yingqiang, Hu Yang, Cheng Yuan. (2020). Application of a resistance Calcium-dependent protein kinase gene  Vp  CDPK13  to powdery mildew. (China Patent Application Serial No. 202010017628.8, Pending)

2. Wen Yingqiang, Hu Yang, Cheng Yuan. (2020). Application of a resistance Calcium-dependent protein kinase gene  Vp  CDPK9  to powdery mildew. (China Patent Application Serial No. 202010017656.X, Pending)

3. Wen Yingqiang, Yu Xuena, Feng Jiayue. (2019). The Magnifier using for laminar flow bench. (China Patent No. ZL201920472181.6)

4. Wen Yingqiang, Han Yongtao, Gao Yurong. (2017). Rapidly evaluated disease resistance inoculated with powdery mildew isolate NAFU1 in grapevine using detached leaves. (China Patent No. ZL201410541569.9)

5. Wen Yingqiang, Han Yongtao, Gao Yurong. (2014). cDNA sequence and application of a specific resistance to powdery mildew gene  VpR8H-1 . (China Patent Application Serial No. 201410541935.0, Pending)

6.Publications (* Correspondence author)

1)  Jie Liu, Mengxue Liu, Liqiang Tan, Baoyu Huai, Xianfeng Ma, Qinglin Pan, Peijing Zheng, Yingqiang Wen, Qiong Zhang, Qi Zhao, Zhensheng Kang*, Shunyuan Xiao. (2021) AtSTP8, an endoplasmic reticulum-localized monosaccharide transporter from Arabidopsis, is recruited to the extrahaustorial membrane during powdery mildew infection. New Phytologist.

https://doi.org/10.1111/nph.17347

2) Yu-Lian Li, Dan-Juan Wang, Yang-Yang Ma, Xiao-Lin Cai, Shunyuan Xiao, Ying-Qiang Wen*, Jia-Yue Feng*. (2021) First Report of  Xanthomonas fragariae  strain YL19 causing crown infection pockets in strawberry in Liaoning Province, China. Plant Disease, DOI: 10.1094/PDIS-12-20-2560-PDN.

3) Yang Hu, Yuan Cheng, Xuena Yu, Jie Liu, Lushan Yang, Yurong Gao, Guihua Ke, Min Zhou, Bo Mu, Shunyuan Xiao, Yuejin Wang, Ying-Qiang Wen*. (2021) Overexpression of two CDPKs from wild Chinese grapevine enhances powdery mildew resistance in  Vitis vinifera  and  Arabidopsis . New Phytologist.

https://doi.org/10.1111/nph.17285

4)  Dong-Yan Wan, Ye Guo, Yuan Cheng, Yang Hu, Shunyuan Xiao, Yuejin Wang, Ying-Qiang Wen*. (2020) CRISPR/Cas9-mediated mutagenesis of  VvMLO3  results in enhanced resistance to powdery mildew in grapevine ( Vitis vinifera ). Horticulture Research, 7(1):116.

5) Yang Lushan, Guo Ye, Hu Yang, Wen Yingqiang*. (2020) CRISPR/Cas9-mediated Mutagenesis of  VviEDR2  Results in Enhanced Resistance to Powdery Mildew in Grapevine ( Vitis vinifera ). Acta Horticulturae Sinica, 47(4): 623-634. (In Chinese, Abstract in English)

6)  Yi-Jie Gou,Yu-Lian Li, Pin-Pin Bi, Dan-Juan Wang, Yang-Yang Ma, Yang Hu, Hou-Cheng Zhou, Ying-Qiang Wen, Jia-Yue Feng*. (2020) Optimization of the protoplast transient expression system for gene functional studies in strawberry ( Fragaria vesca ). Plant Cell Tiss Organ Cult, 141(1): 41-53.

7)  Yin-Ge Xie, Yang-Yang Ma, Pin-Pin Bi, Wei Wei, Jie Liu, Yang Hu, Yi-Jie Gou, Dong Zhu, Ying-Qiang Wen, Jia-Yue Feng*. (2020) Transcription factor  FvTCP9  promotes strawberry fruit ripening by regulating the biosynthesis of abscisic acid and anthocyanins. Plant Physiology and Biochemistry, 146: 374-383.

8) Yang Hu, Yu-Rong Gao, Lu-Shan Yang, Wei Wang, Yue-Jin Wang, Ying-Qiang Wen*. (2019) The cytological basis of powdery mildew resistance in wild Chinese  Vitis  species. Plant Physiology and Biochemistry, 144: 244-253.

9)  Jie Liu, Feng-Li Zhao, Ye Guo, Xiu-cai Fan, Yue-jin Wang, Ying-Qiang Wen*. (2019) The ABA receptor-like gene  VyPYL9  from drought-resistance wild grapevine confers drought tolerance and ABA hypersensitivity in  Arabidopsis . Plant Cell Tiss Organ Cult, 138(3): 543-558.

10) Guo Ye, Wan Dongyan, Chai Zhuangzhuang, Wang Yuejin, Wen Yingqiang*. (2019) Knock-out Analysis of  VviPDS1  Gene Using CRISPR/Cas9 in Grapevine, Acta Horticulturae Sinica, 46 (4): 623-634. (In Chinese, Abstract in English)

11） Yang Hu, Yajuan Li, Fengjuan Hou, Dongyan Wan, Yuan Cheng, Yongtao Han, Yurong Gao, Jie Liu, Ye Guo, Shunyuan Xiao, Yuejin Wang, Ying-Qiang Wen*. (2018) Ectopic expression of Arabidopsis broad-spectrum resistance gene  RPW8.2  improves the resistance to powdery mildew in grapevine ( Vitis vinifera ). Plant Science, 267: 20–31.

12)  Cheng Yuan, Wan Dongyan, Zhang Baolin, Wang Yuejin, Wen Yingqiang*. (2017) Induction, preservation, propagation and cytological observation of pro-embryonic masses of grapevine, Journal of Fruit Science, 34(8): 968-976. (In Chinese, Abstract in English)

13) Yu-Rong Gao, Yong-Tao Han, Feng-Li Zhao, Ya-Juan Li, Yuan Cheng, Qin Ding, Yue-Jin Wang, Ying-Qiang Wen*. (2016) Identification and utilization of a new  Erysiphe necator  isolate NAFU1 to quickly evaluate powdery mildew resistance in wild Chinese grapevine species using detached leaves. Plant Physiology and Biochemistry, 98: 12-24.

14) Yang Hu, Yong-Tao Han, Kai Zhang, Feng-Li Zhao, Ya-Juan Li, Yi Zheng, Yue-Jin Wang, Ying-Qiang Wen*. (2016) Identification and expression analysis of heat shock transcription factors in the wild Chinese grapevine ( Vitis pseudoreticulata ). Plant Physiology and Biochemistry, 99: 1-10.

15) Feng-Li Zhao, Ya-Juan Li, Yang Hu, Yu-Rong Gao, Xue-Wen Zang, Qin Ding, Yue-Jin Wang, Ying-Qiang Wen*. (2016) A highly efficient grapevine mesophyll protoplast system for transient gene expression and the study of disease resistance proteins. Plant Cell Tiss Organ Cult, 125: 43-57.

16) Xianfeng Ma, Wen-Ming Wang, Florian Bittner, Nadine Schmidt, Robert Berkey, Lingli Zhang, Harlan King, Yi Zhang, Jiayue Feng, Yingqiang Wen, Liqiang Tan, Yue Li, Qiong Zhang, Ziniu Deng, Xingyao Xiong, Shunyuan Xiao*. (2016) Dual and Opposing Roles of Xanthine Dehydrogenase in Defense-Associated Reactive Oxygen Species Metabolism in Arabidopsis. The Plant Cell, 28(5): 1108-1126.

17) Kai Zhang, Yong-Tao Han, Feng-Li Zhao, Yang Hu, Yu-Rong Gao, Yan-Fei Ma, Yi Zheng,Yue-Jin Wang and Ying-Qiang Wen*. (2015) Genome-wide Identification and Expression Analysis of the CDPK Gene Family in Grape,  Vitis  spp. BMC Plant Biology, 15:164.

18) Ma Hui, Han Yong-tao, Gao Yu-rong, Xu Hong, Wang Yue-jin, Wen Ying-qiang*. (2015) Molecular cloning and expression analysis of  RPW8.2  homologous genes in grapevine, Journal of Northwest forestry University, 30(1):60-68. (In Chinese, Abstract in English)

19) Ma Yanfei, Liu Xiaohui, Wen Yingqiang*. (2015) Application effects of three new foliar-fertilizers on grapevine, Sino-Overseas Grapevine & Wine, 3: 28-31. (In Chinese, Abstract in English)   

20) Wenming Wang, Yi Zhang, Yingqiang Wen, Robert Berkey, Xianfeng Ma, Zhiyong Pan, Dipti Bendigeri, Harlan King, Qiong Zhang, and Shunyuan Xiao. (2013) A comprehensive mutational analysis of the Arabidopsis resistance protein RPW8.2 reveals key amino acids for defense activation and protein targeting. The Plant Cell, 25(10): 4242-4261.

21) Yingqiang Wen, Xiping Wang, Shunyuan Xiao, Yuejin Wang. (2012) Ectopic expression of  VpALDH2B4 , a novel Aldehyde Dehydrogenase gene from Chinese wild grapevine ( Vitis pseudoreticulata ), enhances resistance to mildew pathogens and salt stress in  Arabidopsis . Planta, 236(2):525-39.

22) Yingqiang Wen, Wenming Wang, Jiayue Feng, Ming-Cheng Luo, Kenichi Tsuda, Fumiaki Katagiri, Gary Bauchan and Shunyuan Xiao. (2011) Identification and utilization of a sow thistle powdery mildew as a poorly adapted pathogen to dissect post-invasion nonhost resistance mechanisms in  Arabidopsis . Journal of Experimental Botany, 62, 2117-2129.

23) Wenming Wang, Robert Berkey, Yingqiang Wen, and Shunyuan Xiao. (2010) Accurate and adequate spatiotemporal expression and localization of RPW8.2 is key to activation of resistance at the host-pathogen interface. Plant signaling & behavior, 5:8, 1002-1005.

24) Wenming Wang, Yingqiang Wen, Robert Berkey, Shunyuan Xiao. (2009) Specific targeting of RPW8.2 to the interfacial membrane encasing the fungal haustorium renders broad-spectrum resistance to powdery mildew in  Arabidopsis . The Plant Cell, 21, 2898-2913.

25) Wen Yingqiang, Feng Jiayue, Wan Chunyan. (2007) Effects of mediums at different ratio on the rooting of seedless grapevine ( Vitis vinifera  L.) cuttings. Sino-Overseas Grapevine & Wine, 3: 9-11. (In Chinese, Abstract in English)  

26) Wen Yingqiang, Feng Jiayue, Wan Chunyan. (2007) The relationship between development, rooting of tissue cultured grapevine plantlets different node and the activity of POD and IAA oxidase. Plant Physiology Communications, 40(4): 723-724. (In Chinese)  

27) Wen Yingqiang, Feng Jiayue, Ren Xiaolin. (2007) Mechanism for prolonging cold storage life of Li jujube ( Zizyphus jujuba  Mill. cv. Lizao) fruit by heat air treatment. Transactions of the Chinese Society of Agricultural Engineering, 23(12):24-29 (In Chinese, Abstract in English)    

28) Ying-qiang Wen, Jia-yue Feng, Xiao-lin Ren. (2007) Effect of hot air treatment on softening and quality of postharvest Li Jujube fruits during cold storage. Acta Agriculturae Boreali-occidentalis Sinica, 16(6):133-136. (In Chinese, Abstract in English)    

29) Wen Yingqiang, Feng Jiayue, Wan Chunyan. (2007) Effects of ABT and CaCN₂ on Budding, Rooting and Some Physiological and Biochemical Metabolize of Grape ( Vitis  vinifera  L. Cv. Thompson seedless) Cuttings. Chinese Agricultural Science Bulletin, 23(12): 270-273. (In Chinese, Abstract in English)  

30) Wen Ying-qiang, Ma Feng-wang. An advance in application and study of apple bagging technology in China. (2006) Journal of Northwest Sci-Tech University of Agriculture and Forestry (Natural Science Edition). 34(2): 100-104. (In Chinese, Abstract in English)

31) Yingqiang Wen, Yali Liu, Ronghua Wang, Yongquan Xu. (2005) 6-BA and PP-333 effects in shelf--life improvement of ( Tulipa gesneriana ) cut flowers. Acta Botanica Boreali-occidentalia Sinica, 25(12): 2535-2538. (In Chinese, Abstract in English)  

32) FENG Jia-yue, ZOU Zhi-rong, WEN Ying-qiang, CHEN Xiu-bin. (2004) Effects of IBA and NAA in different media on cuttage rooting of  Petunia hybrida . Journal of Northwest Sci-Tech University of Agriculture and Forestry (Natural Science Edition). 32(8): 34-36. (In Chinese, Abstract in English)

33) Ying-qiang Wen, Xiao-lin Ren, Feng-wang Ma, Jia-yue Feng. (2002) Affection of Heat Treatment on Cold Storage Effect and Quality of  Zizyphus Jujuba  Mill. Acta Agriculturae Boreali-occidentalis Sinica,11(4): 81-83. (In Chinese, Abstract in English)